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untagged human parkin  (Addgene inc)


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    Addgene inc untagged human parkin
    Untagged Human Parkin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/untagged+human+parkin/pmc12335601-448-8-12?v=Addgene+inc
    Average 93 stars, based on 10 article reviews
    untagged human parkin - by Bioz Stars, 2026-07
    93/100 stars

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    untagged human parkin  (Addgene inc)
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    Addgene inc untagged human parkin
    Untagged Human Parkin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/untagged+human+parkin/pmc12335601-448-8-12?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    untagged human parkin - by Bioz Stars, 2026-07
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    e3 untagged parkin e3 ubiquitin ligase  (Boston Biochem)
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    Boston Biochem e3 untagged parkin e3 ubiquitin ligase
    Primary assay development (A) Schematic of the biochemical assay including detection by an FK2 antibody, which recognizes polyubiquitin and provides an FRET signal to the FITC-labeled <t>ubiquitin.</t> The components of the reaction are depicted with circles. (B) Comparison of the FRET ratio of the WT-, W403A- and C431S-Parkin after 1 h incubation. (C) Silver stain indicating that W403A-Parkin was more active than WT-Parkin in the presence of 1 μM pUb, as indicated by the disappearance of the Parkin band and the appearance of a high-molecular weight smear. (D) Dependency of WT- (blue) and W403A-Parkin (red) activity on pUb concentration. The C431S-Parkin (yellow) is inactive at all pUb concentrations. (E) Titration of pUb in TR-FRET assay with WT-Parkin (blue) at a 3 h reaction time and calculation of Ec 50 . Each data point represents 2 technical replicates. (F) At a given time (i.e., 180 min), we can enable a large window between WT-Parkin (blue) and the W403A-Parkin positive control (red). Each data point represents 2 technical replicates. Statistical test performed: ANOVA with Dunnett’s MCT versus WT ∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. For panel B, error bars represent standard deviation with n = 3 technical replicates. See also .
    E3 Untagged Parkin E3 Ubiquitin Ligase, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/untagged+human+parkin/pmc08733272-53-0-7?v=Boston+Biochem
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    e3 untagged parkin e3 ubiquitin ligase - by Bioz Stars, 2026-07
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    parkin cdna clones  (OriGene)
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    Primary assay development (A) Schematic of the biochemical assay including detection by an FK2 antibody, which recognizes polyubiquitin and provides an FRET signal to the FITC-labeled <t>ubiquitin.</t> The components of the reaction are depicted with circles. (B) Comparison of the FRET ratio of the WT-, W403A- and C431S-Parkin after 1 h incubation. (C) Silver stain indicating that W403A-Parkin was more active than WT-Parkin in the presence of 1 μM pUb, as indicated by the disappearance of the Parkin band and the appearance of a high-molecular weight smear. (D) Dependency of WT- (blue) and W403A-Parkin (red) activity on pUb concentration. The C431S-Parkin (yellow) is inactive at all pUb concentrations. (E) Titration of pUb in TR-FRET assay with WT-Parkin (blue) at a 3 h reaction time and calculation of Ec 50 . Each data point represents 2 technical replicates. (F) At a given time (i.e., 180 min), we can enable a large window between WT-Parkin (blue) and the W403A-Parkin positive control (red). Each data point represents 2 technical replicates. Statistical test performed: ANOVA with Dunnett’s MCT versus WT ∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. For panel B, error bars represent standard deviation with n = 3 technical replicates. See also .
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    mouse parkin  (OriGene)
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    Primary assay development (A) Schematic of the biochemical assay including detection by an FK2 antibody, which recognizes polyubiquitin and provides an FRET signal to the FITC-labeled <t>ubiquitin.</t> The components of the reaction are depicted with circles. (B) Comparison of the FRET ratio of the WT-, W403A- and C431S-Parkin after 1 h incubation. (C) Silver stain indicating that W403A-Parkin was more active than WT-Parkin in the presence of 1 μM pUb, as indicated by the disappearance of the Parkin band and the appearance of a high-molecular weight smear. (D) Dependency of WT- (blue) and W403A-Parkin (red) activity on pUb concentration. The C431S-Parkin (yellow) is inactive at all pUb concentrations. (E) Titration of pUb in TR-FRET assay with WT-Parkin (blue) at a 3 h reaction time and calculation of Ec 50 . Each data point represents 2 technical replicates. (F) At a given time (i.e., 180 min), we can enable a large window between WT-Parkin (blue) and the W403A-Parkin positive control (red). Each data point represents 2 technical replicates. Statistical test performed: ANOVA with Dunnett’s MCT versus WT ∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. For panel B, error bars represent standard deviation with n = 3 technical replicates. See also .
    Mouse Parkin, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Primary assay development (A) Schematic of the biochemical assay including detection by an FK2 antibody, which recognizes polyubiquitin and provides an FRET signal to the FITC-labeled ubiquitin. The components of the reaction are depicted with circles. (B) Comparison of the FRET ratio of the WT-, W403A- and C431S-Parkin after 1 h incubation. (C) Silver stain indicating that W403A-Parkin was more active than WT-Parkin in the presence of 1 μM pUb, as indicated by the disappearance of the Parkin band and the appearance of a high-molecular weight smear. (D) Dependency of WT- (blue) and W403A-Parkin (red) activity on pUb concentration. The C431S-Parkin (yellow) is inactive at all pUb concentrations. (E) Titration of pUb in TR-FRET assay with WT-Parkin (blue) at a 3 h reaction time and calculation of Ec 50 . Each data point represents 2 technical replicates. (F) At a given time (i.e., 180 min), we can enable a large window between WT-Parkin (blue) and the W403A-Parkin positive control (red). Each data point represents 2 technical replicates. Statistical test performed: ANOVA with Dunnett’s MCT versus WT ∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. For panel B, error bars represent standard deviation with n = 3 technical replicates. See also .

    Journal: iScience

    Article Title: Discovery of small-molecule positive allosteric modulators of Parkin E3 ligase

    doi: 10.1016/j.isci.2021.103650

    Figure Lengend Snippet: Primary assay development (A) Schematic of the biochemical assay including detection by an FK2 antibody, which recognizes polyubiquitin and provides an FRET signal to the FITC-labeled ubiquitin. The components of the reaction are depicted with circles. (B) Comparison of the FRET ratio of the WT-, W403A- and C431S-Parkin after 1 h incubation. (C) Silver stain indicating that W403A-Parkin was more active than WT-Parkin in the presence of 1 μM pUb, as indicated by the disappearance of the Parkin band and the appearance of a high-molecular weight smear. (D) Dependency of WT- (blue) and W403A-Parkin (red) activity on pUb concentration. The C431S-Parkin (yellow) is inactive at all pUb concentrations. (E) Titration of pUb in TR-FRET assay with WT-Parkin (blue) at a 3 h reaction time and calculation of Ec 50 . Each data point represents 2 technical replicates. (F) At a given time (i.e., 180 min), we can enable a large window between WT-Parkin (blue) and the W403A-Parkin positive control (red). Each data point represents 2 technical replicates. Statistical test performed: ANOVA with Dunnett’s MCT versus WT ∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. For panel B, error bars represent standard deviation with n = 3 technical replicates. See also .

    Article Snippet: E3: Untagged Parkin E3 ubiquitin Ligase , Boston Biochem , Cat# E3-160.

    Techniques: Labeling, Ubiquitin Proteomics, Comparison, Incubation, Silver Staining, High Molecular Weight, Activity Assay, Concentration Assay, Titration, Positive Control, Standard Deviation

    Journal: iScience

    Article Title: Discovery of small-molecule positive allosteric modulators of Parkin E3 ligase

    doi: 10.1016/j.isci.2021.103650

    Figure Lengend Snippet:

    Article Snippet: E3: Untagged Parkin E3 ubiquitin Ligase , Boston Biochem , Cat# E3-160.

    Techniques: Ubiquitin Proteomics, Recombinant, Saline, Blocking Assay, Transfection, Cloning, Mutagenesis, Staining, Protease Inhibitor, Nano Differential Scanning Fluorimetry, Adhesive